The FluoFit software package is a powerful analysis software for fluorescence decay and anisotropy measurements. Tail fitting as well as a numerical reconvolution algorithm to account for the finite Instrument Response Function (IRF) can be applied. The decay data can be fitted to exponential decay models up to 4th order or alternatively to different lifetime distribution models (Gaussian, Lorentzian and Stretched Exponential). Global fitting as well as batch mode fitting is supported for all models. The software allows to freely vary the number of fit parameters, including IRF and signal background as well as time shift. Start parameters for the fitting algorithm can be determined automatically or entered manually. The fitting limits are easily adjusted with graphical sliders. Reduced chi-square, weighted residual and autocorrelation trace are shown for assessment of the goodness of fit. An advanced error analysis using different methods is also possible. User preferences are widely adjustable and can be automatically stored and retrieved. The 32-bit software is available for Windows 2000/XP/Vista and features a modern and easy to use graphical user interface. Results can be printed, saved and exported for later reference. The program supports data files from the HydraHarp 400, PicoHarp 300, TimeHarp 200, NanoHarp 250, SPC and MSA TCSPC systems as well as ASCII data files. Instrument response and fluorescence decay may be loaded from different sources. A comprehensive on-line help library is provided for ease of use.
Features Tail fitting or iterative reconvolution, maximum likelihood estimation Exponential decay models up to 4th order Lifetime distribution models (Gaussian, Lorentzian, Stretched Exponentials) Anisotropy analysis with an exponential decay model up to 4th order Global analysis and batch fitting for all models Rigorous error-analysis with three different methods ASCII and direct data import from HydraHarp 400, PicoHarp 300, TimeHarp 200, NanoHarp 250, SPC and MSA data Unlimited number of data points Storage of user preferences 32 Bit Windows 2000/XP/Vista software Easy to use graphical interface